Evy, Yulianti and Anna, Rakhmawati and Kartika Ratna, Pertiwi (2014) ANTIMICROBIAL ACTIVITY OF EXTRA CELULLAR PROTEIN DERIVED FROM SIX ISOLATED THERMOPHILIC BACTERIA. Proceeding of International Conference On Research, Implementation And Education Of Mathematics And Sciences 2014. (Submitted)
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Abstract
Antibiotics are metabolic by-products of complex biosynthetic pathways in microorganisms, some of which are proteins or peptides, known for their bactericidal and/or fungicidal effect on microorganisms. This Antimicrobial Peptides (AMP) produced by certain bacteria are toxic for other bacteria or for fungi but not for mammalia. The aim of this study was to investigate the antimicrobial activity of protein derived from six isolated thermophilic bacteria (113a, 94b, 153, 104c, 83, 110a). Bacteria was fermented in NB medium with 1% glucose in 55⁰C for 24 hr. Protein was isolated from cell-free extract by salting out method with 60% ammonium sulphate followed by dialysis. Antimicrobial activity was tested by growing several pathogenic microbes (Eschericia coli, Staphylococcus aureus, and Candida albican) in NA and PDA medium and then layering the papper disk with protein extract onto the top of those pathogenic microbes. Protein which exhibits antimicrobial activity was shown by the presence of a clear zone around the papper disk. The result of this study showed that almost protein derived from six isolated thermophilic bacteria demonstrate antimicrobial activity to Eschericia coli, Staphylococcus aureus, and Candida albican, except the ones isolated from thermophilic bacteria no 104c and 83, which have no antimicrobial effect to Eschericia coli.
| Item Type: | Article |
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| Uncontrolled Keywords: | thermophilic, bacteria, antimicrobial peptide |
| Subjects: | Prosiding > ICRIEMS 2014 > BIOLOGY & BIOLOGY EDUCATION |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Pendidikan Biologi > Biologi |
| Depositing User: | Eprints |
| Date Deposited: | 06 Nov 2014 02:03 |
| Last Modified: | 06 Nov 2014 02:03 |
| URI: | http://eprints.uny.ac.id/id/eprint/11338 |
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