Arianingrum, Retno and Sunarminingsih, Retno and Meiyanto, Edy and Mubarika, Sofia CYTOTOXIC EFFECT OF PARA HYDROXY META METHOXY CHALCONE (pHmMC) ON MCF-7 BREAST CANCER CELLS BY INDUCING CELL ARREST AND APOPTOSIS. Proceeding of International Conference On Research, Implementation And Education Of Mathematics And Sciences 2015. ISSN 978-979-96880-8-8
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Abstract
Chalcones (1,3-diphenylpropen-1-ones), a biosynthetic product of the shikimate pathway, belonging to flavanoid family are precursors of open chain flavonoids and isoflavonoids, which are abundant in edible plants. These compounds have been studied as therapeutics, especially as antitumor drugs. A chalcone derivate, para hydroxy metha methoxy chalcone (pHmMC) or 3 - (4'-hydroxy-3'- methoxyphenyl)-1-phenyl-2-propene-1-on was synthesized by reaction between vanillin and acetofenon through cross-aldol condensation reaction under acidic conditions. The aim of this study is to investigate the cytotoxic effect of pHmMC on MCF7 breast cancer cells and its mechanism through investigation on apoptosis and cell cycle arrest. The cytotoxic effect on MCF-7 was analyzed by MTT [3-4, 5 dimetiltiazol-2-yl)-2,5difeniltetrazoilium bromide] assay. Flowcytometry method was used to determine the influence of pHmMC in the regulation of cell cycle arrest and apoptosis.The result showed that the pHmMC inhibited MCF-7 cell growth with IC50 40 M. The cytotoxic activity is influenced by the ability of pHmMC to induce apoptosis and G2/M arrest.
| Item Type: | Article |
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| Uncontrolled Keywords: | pHmMC, cytotoxic effect, apoptosis, cell cycle arrest, and MCF-7 breast cancer cells |
| Subjects: | Prosiding > ICRIEMS 2015 > Chemistry & Chemistry Education Prosiding |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Jurusan Pendidikan Kimia > Kimia |
| Depositing User: | Seminar |
| Date Deposited: | 23 Jun 2015 05:51 |
| Last Modified: | 23 May 2019 04:50 |
| URI: | http://eprints.uny.ac.id/id/eprint/21235 |
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