Lumbung Pustaka UNY: No conditions. Results ordered -Date Deposited. 2024-03-29T08:39:38ZEPrintshttp://eprints.uny.ac.id/apw_template/images/sitelogo.pnghttps://eprints.uny.ac.id/2012-08-07T01:45:05Z2019-01-29T15:24:14Zhttp://eprints.uny.ac.id/id/eprint/2912This item is in the repository with the URL: http://eprints.uny.ac.id/id/eprint/29122012-08-07T01:45:05ZSTUDI KARAKTERISASI ENZIM AMILASE URIN
MANUSIA TERHADAP PATITujuan dari penelitian ini adalah untuk mengident] tikasi adanya aktivitas enzim amilase dalam urin manusia dan menentukan besar aktivitasnya dengan enzim a-amilase murni sebagai pembanding. Mengetahui ada tidaknya perbedaan ektivitas antara enzim amilase urin enzim a-amilase mzrrnl.
Subjek dalam penelitian ini ada(ah pati kentang dan objeknya adalah aktivitas enzim amilase urin manusia dan enzim cz-amilwSe munii yang digambarkan sebagai kadar glukosa hasil hidrolisis dari pati kentang. Penentuan
"zkterisasi aktivitas ellzim afnilase dilakukwi nlelalul dua 1aj)gkab . Langkah petrtama yaita uji kuah tak enzim atmlase dengart metode Wohlgemccth darr cry kualitatif glukosa dengan ujj Mo1ish dan uji Benedict. Langkab kedua yaitu
penentuan kondisi optimum aktivitas enzim amilase urine dan enzim a-amilase murnt; yang meliputi penentuan waktu inkubasi, ptt, dan suhu optimum dan meaentuan aktivr'tasnya pada variasi konsentrasl substrat 0,25; 0,50, D, 7_5; 1; 1,50; 2 % b/v.
Hasil penelitian menunjukkan bahwa ada aktivitas enzim amilase urin manusia terhadap pati kentang dengan enzim a-amilase murni sebagai pembanding, besarnya aktivitas enzim a-amilase mw-ni pada variasi konsentrasi substrat 0,25; 0,50; 0,75; 1; 1,50; 2 % b/v adalah sebesar 1,823x107; 1,883x10~; 1,928x10~; 1,98x10l-l 2,25x10l; 2,233x10'l mg.mh"imenit ', dan untuk enzim amilase urin ada(ah l, 853; 2,148; 2,2i; 2,493; 2,57; 2,788 m.m1,"Imenit"I. Hasil tersebut juga menunjukkan bahwa ada perbedaan aktivitas enzim amilase urin manusia dan enzim a-amilase mutniBudiati Desti2012-08-07T01:44:58Z2019-01-29T15:24:25Zhttp://eprints.uny.ac.id/id/eprint/2917This item is in the repository with the URL: http://eprints.uny.ac.id/id/eprint/29172012-08-07T01:44:58ZA STUDY ON CHARACTERIZATION OF HUMAN URINE AMYLASE ENZYME ON STARCHThe research aims at identifying amylase enzyme activities in human urine, determining the amounts of the activities with pure a-amylase enzyme as a comparison, and determining whether there was any difference between urine amylase enzyme and pure a-amylase enzyme.
The research subject was potato starch and the objects were human urine amylase enzyme activity and pure a-amylase enzyme described as glucose levels of the hydrolysis of potato starch. Determination of amylase enzyme activities was done through two steps. The first step was a qualitative test of the amylase enzyme using Wohlgemuth method and the qualitative glucose test using Molish test and Benedict test. The second step was determining the optimum condition of the urine amylase enzyme activities and a-amylase pure enzyme activities involving determining the incubation time, pH, and the optimal temperature and determining the activities on substrate concentration variations of 0.25 % (w /v), 0.50 % (w /v), 0.75 % (w /v), 1 % (w /v), 1.50 % (w /v), and 2 % (w /v).
The results showed that there were human urine amylase enzyme activities on potato starch using pure a-amylase enzyme as a comparison. The amounts of pure a-amylase enzyme activities on the variation of substrate concentrations of 0.25 %, 0.50 %, 0.75 %, 1 %, 1.50 %, and 2 % (w/v) amounted to 1.823 x 10', 1.883 x 10', 1.928 x 10', 1.98 x 10', 2.25 x 10', and 2.233 x 10' mg/ml.minute while the amounts of urine amylase enzyme activities were l.853 x 10', 2.148 x 10', 2.21 x 10', 2.493 x 10', 2.57 x 10', and 2.788 x 10' mg/ml.minute. The results also showed that there were differences in human urine amylase enzyme and pure a-amylase enzyme activities. Budiati Desti