Lumbung Pustaka UNY: No conditions. Results ordered -Date Deposited. 2024-03-28T09:40:04ZEPrintshttp://eprints.uny.ac.id/apw_template/images/sitelogo.pnghttps://eprints.uny.ac.id/2014-11-06T04:40:52Z2014-11-06T04:40:52Zhttp://eprints.uny.ac.id/id/eprint/11381This item is in the repository with the URL: http://eprints.uny.ac.id/id/eprint/113812014-11-06T04:40:52ZPRODUCTION AND CHARACTERIZATION OF ANTI FIM-C Salmonella typhi
NATIVE PROTEIN ANTIBODY IN DDY MICETyphoid fever is a disease affect many people in developing countries including
Indonesia. Prevention can be aided by vaccination to the bacteria; however it still needs
further research. This research is aimed to determine the immunogenicity of fim-C native
protein Salmonella typhi in vivo as a vaccine candidate in ddY mice. Experimental
method is used in this research. This research used antigen of pure fim-C native
recombinant protein from its previous research. The ddY mice are categorized into five
groups, namely experiment group 1 (protein Fim-C+Freund's Complete/Incomplete
Adjuvant), experiment group 2 (protein Fim-C S. typhi), control group 1 (FCA/FIA
adjuvants), control group 2 (PBS1x), and normal group (PBS1x). The results showed that
the ddY mice produced antibody response after subcutaneous injection of the Fim-C S.
typhi native protein +adjuvant or without adjuvant. The antibody responses with Fim-C S.
typhi native protein as antigen+adjuvant gave higher absorbans than the Fim-C antigen
without adjuvant. It is also showed that the antibody titer from the first to fourth injection
has gradually increased. These data showed that Fim-C S. Typhi native protein had higher
immunogenicity. It is concluded that the Fim-C S. typhi native protein can be used as a
potential recombinant vaccine candidate against typhoid fever.Nurjayadia MuktiningsihHasana UmarApriyania DeaDewia Fera KurniaKartika Irma RatnaPuspasari FernitaNatalia Dessy