%0 Journal Article
%A Evy, Yulianti
%A Anna, Rakhmawati
%A Kartika Ratna, Pertiwi
%D 2014
%F UNY:11338
%I Yogyakarta State University
%J Proceeding of  International Conference On Research, Implementation And Education  Of Mathematics And Sciences 2014
%K thermophilic, bacteria,  antimicrobial peptide
%T ANTIMICROBIAL ACTIVITY OF EXTRA CELULLAR PROTEIN   DERIVED FROM SIX ISOLATED THERMOPHILIC BACTERIA
%U http://eprints.uny.ac.id/11338/
%X Antibiotics are metabolic by-products of complex biosynthetic pathways in microorganisms, some of which are proteins or peptides, known for their bactericidal and/or fungicidal effect on microorganisms.  This Antimicrobial Peptides (AMP) produced by certain bacteria are toxic for other bacteria or for fungi but not for mammalia. The aim of this study was to investigate the antimicrobial activity of protein derived from six isolated thermophilic bacteria (113a, 94b, 153, 104c, 83, 110a). Bacteria was fermented in NB medium with 1% glucose in 55⁰C for 24 hr. Protein was isolated from cell-free extract by salting out method with 60% ammonium sulphate followed by dialysis. Antimicrobial activity was tested by growing several pathogenic microbes (Eschericia coli, Staphylococcus aureus, and Candida albican) in NA and PDA medium and then layering the papper disk with protein extract onto the top of those pathogenic microbes. Protein which exhibits antimicrobial activity was shown by the presence of a clear zone around the papper disk. The result of this study showed that almost protein derived from six isolated thermophilic bacteria demonstrate antimicrobial activity to Eschericia coli, Staphylococcus aureus, and Candida albican, except the ones isolated from thermophilic bacteria no 104c and 83, which have no antimicrobial effect to  Eschericia coli.