TY  - JOUR
UR  - http://fmipa.uny.ac.id
AV  - public
KW  - optimization 
KW  -  PCR 
KW  -  SSRs primary
KW  -  amplification
TI  - OPTIMIZATION  OF  PCR CONDITIONS FOR AMPLIFY  
MCROSATELLITE LOCI IN COTTON  (Gossypium hirsutum ) DNA
ID  - UNY11329
A1  - Dede, Nuraida
PB  - Yogyakarta State University
Y1  - 2014/05//
N2  - Optimization of polymerase chain Reactian ( PCR ) condition has been performed to amplification of genomic DNA for cotton Gossypium hirsutum. The optimization using  5 SSR primers pairs from JESPR  series, that is JESPR 247, JESPR 280, JESPR 292, JESPR 299, and JESPR 303. The aim of optimization is to obtain the right conditions for amplification through PCR reaction. The course Optimization includes, annealing temperature, the amount of DNA template, and the amount of PCR mix. The results showed that the amount of DNA template 2.5 µl of total volume of 22 µl , and the volume of 12.5 µl PCR mix, an optimal volume to deliver a good amplification product. Annealing temperature of 480C was the optimum temperature for the primary JESPR 280, JESPR 292, JESPR 299, and JESPR 303, while for the JESPR 247 primary optimum temperature is 500C. .
JF  - Proceeding of  International Conference On Research, Implementation And Education  Of Mathematics And Sciences 2014
ER  -